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KMID : 0880220130510030373
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Journal of Microbiology
2013 Volume.51 No. 3 p.373 ~ p.379
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Influence of culture conditions and medium composition on the production of antibacterial compounds by marine Serratia sp. WPRA3
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Mahtab Jafarzade
Nur Ain Yahya
Asmat Ahmad
Fatemeh Shayesteh
Gires Usup
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Abstract
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This study was undertaken to investigate the influence of culture conditions and medium components on production of antibacterial compounds by Serratia sp. WPRA3 (JX020764) which was isolated from marine water of Port Dickson, Malaysia. Biochemical, morphological, and molecular characteristics suggested that the isolate is a new candidate of the Serratia sp. The isolate showed strong antimicrobial activity against fungi, Gram-negative and Gram-positive bacteria. This bacterium exhibited optimum antibacterial compounds production at 28°C, pH 7 and 200 rev/min aeration during 72 h of incubation period. Highest antibacterial activity was obtained when sodium chloride (2%), yeast extract (0.5%), and glucose concentration (0.75%) were used as salt, nitrogen, and carbon sources respectively. Different active fractions were obtained by Thin-Layer Chromatography (TLC) and Flash Column Chromatography (FCC) from ethyl acetate crude extracts namely OCE and RCE in different culture conditions, OCE (pH 5, 200 rev/min) and RCE (pH 7/without aeration). In conclusion, the results suggested different culture conditions have a significant impact on the types of secondary metabolites produced by the bacterium.
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KEYWORD
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optimization, antibacterial compound, pigmented bacteria, marine bacteria, Serratia
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